Strain Data Sheet

RBRC04608

Strain Information

Image
BRC No.RBRC04608
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Runx1<tm1Homy>
Former Common nameRunx1-delta IRES
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2008.
Strain description
Colony maintenance
References
A 5' untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model.
Nagamachi A, Htun P W, Ma F, Miyazaki K, Yamasaki N, Kanno M, Inaba T, Honda Z, Okuda T, Oda H, Tsuji K, Honda H
Dev. Biol., 345, 226-236 (2010). 20647008

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)16EGFP
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)
    • Frtyeast FRT (flippase recombination target) site16Frt
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)
    • Iresinternal ribosomal entry site (EMCV)16
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)
    • RUNX1runt related transcription factor 1(human)16RUNX1
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)
    • Runx1runt related transcription factor 116Runx1targeted mutation 1.1, Hiroaki Honda
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)
    • loxPphage P1 loxP16loxP
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)
    • loxPphage P1 loxP16loxP
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • hereditary thrombocytopenia and hematological cancer predisposition syndrome associated with RUNX1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal common myeloid progenitor cell morphology(MP:0006410)

  • abnormal megakaryocyte progenitor cell morphology(MP:0002413)

  • abnormal pericyte morphology(MP:0003719)

  • aneurysm(MP:0003279)

  • decreased erythroid progenitor cell number(MP:0008973)
    • more 7 phenotypes
  • decreased hematopoietic cell number(MP:0011182)

  • dilated vasculature(MP:0004938)

  • hemorrhage(MP:0001914)

  • impaired hematopoiesis(MP:0001606)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • pale liver(MP:0000603)

  • skin edema(MP:0001786)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, human RUNX1B cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Jellyfish GFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse Runx1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data