Strain Data Sheet

RBRC04606

Strain Information

Image
BRC No.RBRC04606
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(Kcnc1-GCaMP2)929Bsi
Former Common namepKv3.1-GCaMP2#929
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Thomas Knoepfel, RIKEN Brain Science Institute. C57BL/6, DBA/2 and ICR mixed background.
Strain descriptionKcnc1-GCaMP2 transgenic mice. Transgene composed of regulatory sequences of rat Kv3.1 (Kcnc1) and the gene for the calcium sensor GCaMP2.
Colony maintenance
References
Activation of cerebellar parallel fibers monitored in transgenic mice expressing a fluorescent Ca2+ indicator protein.
Javier Díez-García, Shinichi Matsushita, Hiroki Mutoh, Junichi Nakai, Masamichi Ohkura, Jennifer Yokoyama, Dimitar Dimitrov, Thomas Knöpfel
Eur. J. Neurosci., 22, 627-635 (2005). 16101744

Optical imaging of postsynaptic odor representation in the glomerular layer of the mouse olfactory bulb.
Fletcher M L, Masurkar A V, Xing J, Imamura F, Xiong W, Nagayama S, Mutoh H, Greer C A, Knopfel T, Chen W R
J. Neurophysiol., 102, 817-830 (2009). 19474178

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • progressive myoclonic epilepsy type 7(MedGEN)
    • GCaMP2RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2rat Kv3.1 (Kcnc1) promoter
  • progressive myoclonic epilepsy type 7(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat Kv3.1 (Kcnc1) genomic DNA, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Simian virus SV40 polyA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur J. Neurosci., 22, 627-635 (2005). J. Neurophysiol., 102, 817-830 (2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorThomas Knöepfel (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Contact Information
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data