Strain Data Sheet

RBRC04553

Information1

Image
BRC No.RBRC04553
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rrp8<tm1Jyan>/2Rbrc
Former Common nameNML-KO, B6.Cg-Nml<tm1>/2
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Akiko Murayama and Jun Yanagisawa, Life Science Center of Tsukuba Advanced Research Alliance, The University of Tsukuba in 2008. iTL ES cells derived from (C57BL/6 x 129)F1 were used. The mutant mice were crossed to C57BL/6 over 6 times.
Strain descriptionNml (Nucleomethylin) gene knockout mice.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesCell Rep., 7(3)807-820 (2014). 24746822
Biochem. Biophys. Res. Commun., 390, 591-596 (2009). 19819226
Cell, 133, 627-639, (2008). 18485871

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rrp8ribosomal RNA processing 8, methyltransferase, homolog (yeast)7Rrp8<tm1Jyan>tatgeted mutation 1, Jun Yanagisawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAphage P1 loxP site, Saccharomyces cerevisiae FRT (flipase recombination target) site, mouse Rrp8 (Nml)genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep., 7(3)807-820 (2014). The availability of the BIOLOGICAL RESOURCE is limited to a Recipient of a non- for-profit organization for a not-for-profit research.
DepositorJun Yanagisawa (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data