Strain Data Sheet



BRC No.RBRC04550
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Nms<tm1Mko>
Former Common nameNMS-KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Strain developmentDeveloped by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain descriptionNms (Neuromedin S) gene knock-out mice. Exon 8 of the Nms gene was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality. Nmu gene knockout mice (RBRC04549), Nmu and Nms double knockout mice (RBRC04737).
Colony maintenanceHeterozygote × wild-type [or Crossing to C57BL/6JJcl]

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nmsneuromedin S1Nms<tm1Mko>targeted mutation 1, Masayasu Kojima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nms genomic DNA
Research application
Specific Term and ConditionsBecause no paper has been published on neuromedin S knockout mice, the RECIPIENT is requested to contact the DEPOSITOR in publishing the research results obtained by use of the BIOLOGICAL RESOURCE.
DepositorMsayasu Kojima (Kurume University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data