RBRC04549

Information1

Image
BRC No.RBRC04549
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Nmu<tm1Mko>
Former Common nameNMU-KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain descriptionNmu (Neuromedin U) gene knock-out mice. Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms gene knockout mice (RBRC04550), Nmu and Nms double knockout mice (RBRC04737).
Colony maintenanceHeterozygote × wild-type [or Crossing to C57BL/6JJcl]
ReferencesNat. Med., 10, 1067-1073 (2004). 15448684
J. Exp. Med., 202, 217-224 (2005). 16009716

Health Report

Examination Date / Room / Rack2012/06/04Room:3-8Rack:C
2012/04/02Room:3-8Rack:C
2012/02/06Room:3-8Rack:C
2011/12/05Room:3-8Rack:C
2011/10/03Room:3-8Rack:C
2011/08/08Room:3-8Rack:C
2011/07/04Room:4-5Rack:C
2011/05/11Room:4-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nmuneuromedin U5Nmu<tm1Mko>targeted mutation 1, Masayasu Kojima

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nmu genomic DNA
Research applicationMetabolism Research
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMsayasu Kojima (Kurume University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Wallrapp A, Riesenfeld SJ, Burkett PR, Abdulnour RE, Nyman J, Dionne D, Hofree M, Cuoco MS, Rodman C, Farouq D, Haas BJ, Tickle TL, Trombetta JJ, Baral P, Klose CSN, Mahlakõiv T, Artis D, Rozenblatt-Rosen O, Chiu IM, Levy BD, Kowalczyk MS, Regev A, Kuchroo VK.
The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation.
Nature 549(7672) 351-356(2017) 28902842