Strain Data Sheet

RBRC04545

Strain Information

Image
BRC No.RBRC04545
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Rnf135<tm1>
Former Common nameRiplet KO
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strain
Appearance
Strain developmentDeveloped by Hiroyuki Oshiumi, Graduate School of Medicine, Hokkaido University. EGR05 ES cells were used to generate the mutant mice.
Strain descriptionRnf135 (Riplet) gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. 129 background (RBRC04545), C57BL/6 mixed background (RBRC06432).
Colony maintenanceHomozygote x Homozygote
References
Riplet/RNF135, a RING finger protein, ubiquitinates RIG-I to promote interferon-beta induction during the early phase of viral infection.
Oshiumi H, Matsumoto M, Hatakeyama S, Seya T
J. Biol. Chem., 284, 807-817 (2009). 19017631

The ubiquitin ligase Riplet is essential for RIG-I-dependent innate immune responses to RNA virus infection.
Oshiumi H, Miyashita M, Inoue N, Okabe M, Matsumoto M, Seya T
Cell Host Microbe, 8, 496-509 (2010). 21147464

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rnf135ring finger protein 13511Rnf135targeted mutation 1
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased circulating interferon-gamma level(MP:0008578)

  • decreased circulating interleukin-6 level(MP:0008597)

  • decreased interferon-alpha secretion(MP:0008563)

  • decreased interferon-beta secretion(MP:0008565)

  • increased susceptibility to viral infection(MP:0002418)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Rnf135 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 284, 807-817 (2009).
    DepositorHiroyuki Oshiumi (Hokkaido University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data