RBRC04545

Information1

Image
BRC No.RBRC04545
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Rnf135<tm1>
Former Common nameRiplet KO
H-2 Haplotype
ES Cell lineEGR-05 [129S2]
Background strain
Appearance
Strain developmentDeveloped by Hiroyuki Oshiumi, Graduate School of Medicine, Hokkaido University. EGR05 ES cells were used to generate the mutant mice.
Strain descriptionRnf135 (Riplet) gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. 129 background (RBRC04545), C57BL/6 mixed background (RBRC06432).
Colony maintenanceHomozygote x Homozygote
ReferencesJ. Biol. Chem., 284, 807-817 (2009). 19017631
Cell Host Microbe, 8, 496-509 (2010). 21147464

Health Report

Examination Date / Room / Rack2015/08/03Room:3-5Rack:A
2015/06/01Room:3-5Rack:A
2015/04/06Room:3-5Rack:A
2015/02/02Room:3-5Rack:A
2015/01/14Room:3-5Rack:A
2015/01/13Room:3-5Rack:A
2014/12/01Room:3-5Rack:A
2014/10/06Room:3-5Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rnf135ring finger protein 13511Rnf135<tm1>targeted mutation 1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Rnf135 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 284, 807-817 (2009).
DepositorHiroyuki Oshiumi (Hokkaido University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data