RBRC04436

Information1

Image
BRC No.RBRC04436
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Msi2<tm1Okn>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain descriptionMsi2 deficient miceMsi2 deficient mice survive to adult and show poor weight gain, low spontaneous movement and sensory dysfunction, with abnormal PNS development. The cause of the phenotype is thought to be poor neural fiber innervations of specific sensory neuron from DRG to spinal cord. These phenotypes are observed both background (C57BL/6J and CD-1) and detailed analyses were performed in CD-1 background.Msi2 deficient male mice show azoospermia and are infertile. Because the movement of sperm taken from epidermis of Msi2 null male was bad, in vitro fertilization was not successful. Msi2 deficient female mice are fertile; however, probability of pregnancy is poor presumably due to the low spontaneous activity. (All date of Msi2 deficient mice are in preparation by Shibata S. et al)
Colony maintenance
References

Health Report

Examination Date / Room / Rack2014/02/18Room:3-DRack:J
2013/12/16Room:3-DRack:J
2013/10/21Room:3-DRack:J
2013/09/02Room:3-DRack:J
2013/08/26Room:3-DRack:J
2013/08/26Room:3-DRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Msi2Musashi homolog 2 (Drosophila)11Msi2<tm1Okn>targeted mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ApolyA11poly A

Information2

Donor DNAmouse Musashi2 genomic DNA, E. coli Tn5neo gene, mouse PGK promoter, SV40 PolyA
Research applicationCell Biology Research
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP

BRC mice in Publications

No Data