RBRC04435

Information1

Image
BRC No.RBRC04435
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.129(B6)-Msi1<tm1Okn>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain descriptionMsi1 deficient miceC57BL/6J background Msi1 deficient mice frequently died of obstructive hydrocephalus within a couple of month with aberrant cell proliferation around cerebral aqueduct. (Sakakibara S et al. Proc Natl Acad Sci USA. 2002) Both male and female are infertile because of the early death of hydrocephalus.On the other hand, CD-1 (ICR) background Msi1 deficient mice survive to adult without gross anatomical phenotype. Since both male and female are fertile, it is possible to mate between Msi1 null mice. (Unpublished data)
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 99, 15194-15199 (2002). 12407178

Health Report

Examination Date / Room / Rack2012/04/02Room:3-5Rack:C
2012/02/06Room:3-5Rack:C
2011/12/05Room:3-5Rack:C
2011/10/03Room:3-5Rack:C
2011/08/08Room:3-5Rack:C
2011/04/25Room:4-2Rack:D
2011/03/07Room:4-2Rack:D
2011/01/12Room:4-2Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Msi1Musashi homolog 1 (Drosophila)5Msi1

Information2

Donor DNAmouse Musashi1 genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), PGK polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 15194-15199 (2002).For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP

BRC mice in Publications

Wolfe AR1, Ernlund A1, McGuinness W1, Lehmann C1, Carl K1, Balmaceda N1, Neufeld KL2.
Suppression of intestinal tumorigenesis in Apc mutant mice by Musashi-1 deletion.
J. Cell. Sci. (2017) 28082422