Strain Data Sheet


Strain Information

BRC No.RBRC04421
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(FVB)-Tcirg1<tm1.2Ywd>
Former Common namea3 mutant, Tcirg1<delta>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Yoh Wada, Institute of Scientific and Industrial Research, Osaka University in 2008.
Strain descriptionThe gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice. Homozygous mutant mice die within 3-4 weeks after birth.
Colony maintenance
ReferencesJ. Cell Sci., 122, 2504-2513 (2009). 19549681

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tcirg1T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.2Ywd>targeted mutation 1.2, Yoh Wada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Ordering Information

Donor DNAPhage phage P1 loxP site, Mouse Tcirg1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorYoh Wada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Matsumoto N, Nakanishi-Matsui M.
Proton pumping V-ATPase inhibitor bafilomycin A1 affects Rab7 lysosomal localization and abolishes anterograde trafficking of osteoclast secretory lysosomes.
Biochem Biophys Res Commun 510(3) 421-426(2019) 30717974

Matsumoto N, Sekiya M, Sun-Wada GH, Wada Y, Nakanishi-Matsui M.
The lysosomal V-ATPase a3 subunit is involved in localization of Mon1-Ccz1, the GEF for Rab7, to secretory lysosomes in osteoclasts.
Sci Rep 12(1) 8455(2022) 35589873