RBRC04421

Information1

Image
BRC No.RBRC04421
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(FVB)-Tcirg1<tm1.2Ywd>
Former Common namea3 mutant, Tcirg1<delta>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain descriptionThe gene is truncated after exon 13. It contains a single FRT site and a single loxP site in the remaining exon 13-proximal segment of intron 13. Immunoblot analysis fails to detect the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, in liver lysates of homozygous mutant mice.
Colony maintenance
ReferencesJ. Cell. Sci., 122, 2504-2513 (2009). 19549681

Health Report

Examination Date / Room / Rack2011/12/12Room:3-CRack:E
2011/11/02Room:3-CRack:E
2011/10/31Room:3-CRack:E
2011/10/31Room:3-CRack:E
2011/10/17Room:3-CRack:E
2011/08/29Room:3-CRack:E
2011/06/20Room:3-CRack:E
2011/04/20Room:3-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tcirg1T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A319Tcirg1<tm1.2Ywd>targeted mutation 1.2, Yoh Wada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Information2

Donor DNAMouse Tcirg1 genomic DNA, Yeast flipase recombination target sites, Phage phage P1 loxP
Research application
Specific Term and ConditionsThe RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or research in non-profit organization is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorYoh Wada (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data