RBRC04399

Information1

Image
BRC No.RBRC04399
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameaquaporin 4 (GFP)-2 (Acc.No. CDB0758K-1)
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentMasato Yasui, School of Medicine, Keio University/Johns Hopkins University and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology.
Strain descriptionaquaporin 4 gene GFP knock-in mice. EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04397, BALB/c background: RBRC04399), delta Pgk-neo type (#5 ES clone derived)(B6 background: RBRC04364, BALB/c mixed: RBRC04400), delta EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04401, BALB/c mixed: RBRC04402), EGFP-Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04398), delta Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04403).
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAJcl]
ReferencesMol Cell Neurosci, 56, 65-75 (2013). 23428384

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
null

Information2

Donor DNAJellyfish GFP, Mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, Mouse polyA signal, phage P1 loxP, yeast FRT (flipase recombination target) sites
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Masato Yasui (myasui@a3.keio.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0758K-1) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未発表) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data