BRC No.RBRC04395
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Siglec1<tm1(HBEGF)Mtka>
Former Common nameCD169-DTR KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Masato Tanaka, RIKEN Research Center for Allergy and Immunology. R1 ES cells were used. C57BL/6 background.
Strain descriptionCD169 (Siglec1) DTR knock-in mice. HB-EGF is expressed from CD169 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Clin. Invest., 117, 2268-2278 (2007). 17657313

Health Report

Examination Date / Room / Rack2020/04/06Room:3-2Rack:B


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>targeted mutation 1, Masato Tanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBEGFheparin-binding EGF-like growth factor (human)2HBEGF

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP


Donor DNAHuman HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Kenji Kohno, Ph.D. and Masato Tanaka, Ph.D. The recipient shall obtain a prior written approval from the depositor.
DepositorMasato Tanaka (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Dec 2012
Genetic Background

BRC mice in Publications

Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
Inflammation 40(4) 1191-1203(2017) 28466432

Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y.
G-CSF mediates lung injury in mice with adenine-induced acute kidney injury.
Int. Immunopharmacol. 63 1-8.(2018) 30056257