Strain Data Sheet



BRC No.RBRC04395
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Siglec1<tm1(HBEGF)Mtka>
Former Common nameCD169-DTR KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Masato Tanaka, RIKEN Research Center for Allergy and Immunology. R1 ES cells were used. C57BL/6 background.
Strain descriptionCD169 (Siglec1) DTR knock-in mice. HB-EGF is expressed from CD169 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Clin. Invest., 117, 2268-2278 (2007). 17657313
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Health Report

Examination Date / Room / Rack2022/06/13Room:3-2Rack:B


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Siglec1sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>targeted mutation 1, Masato Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)2HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP


Donor DNAHuman HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER (Masato Tanaka, Ph.D. and Kenji Kohno, Ph.D.) is requested.
DepositorMasato Tanaka (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Dec 2012
Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Chalot M, Barroca V, Devanand S, Hoffschir F, Roméo PH, Moreno SG.
Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation.
Blood Adv (2022) 35100346

Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
Inflammation 40(4) 1191-1203(2017) 28466432

Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y.
Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages.
Int Immunopharmacol 78 106012(2020) 31865052

Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y.
G-CSF mediates lung injury in mice with adenine-induced acute kidney injury.
Int Immunopharmacol 63 1-8(2018) 30056257

Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC.
Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
J Clin Invest 129(7) 2920-2931(2019) 31039135

Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI.
Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen.
Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104