Strain Data Sheet

RBRC04395

Strain Information

Image
BRC No.RBRC04395
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Siglec1<tm1(HBEGF)Mtka>
Former Common nameCD169-DTR KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Masato Tanaka, RIKEN Research Center for Allergy and Immunology. R1 ES cells were used. C57BL/6 background.
Strain descriptionCD169 (Siglec1) DTR knock-in mice. HB-EGF is expressed from CD169 locus.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.
M Saito, T Iwawaki, C Taya, H Yonekawa, M Noda, Y Inui, E Mekada, Y Kimata, A Tsuru, K Kohno
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Diphtheria toxin receptor-mediated conditional and targeted cell ablation in transgenic mice.
Saito M, Iwawaki T, Taya C, Yonekawa H, Noda M, Inui Y, Mekada E, Kimata Y, Tsuru A, Kohno K
Nat. Biotechnol., 19, 746-750 (2001). 11479567

Critical role of macrophages in the marginal zone in the suppression of immune responses to apoptotic cell-associated antigens.
Miyake Y, Asano K, Kaise H, Uemura M, Nakayama M, Tanaka M
J. Clin. Invest., 117, 2268-2278 (2007). 17657313

Health Report

Examination Date / Room / Rack2025/03/10Room:3-2Rack:BSentinel mouse program
2024/12/09Room:3-2Rack:BSentinel mouse program
2024/11/06Room:3-2Rack:BAdditional tests
2024/09/09Room:3-2Rack:BSentinel mouse program
2024/06/10Room:3-2Rack:BSentinel mouse program
2024/03/11Room:3-2Rack:BSentinel mouse program
2023/12/11Room:3-2Rack:BSentinel mouse program
2023/09/11Room:3-2Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBEGF heparin-binding EGF-like growth factor (human)2HBEGF
Siglec1
MGI:99668		sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(HBEGF)Mtka>
MGI:3720841		targeted mutation 1, Masato Tanaka
loxP phage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dendritic cell physiology(MP:0002376)

  • abnormal immune system physiology(MP:0001790)

  • abnormal immune tolerance(MP:0005000)

  • abnormal macrophage physiology(MP:0002451)

  • abnormal metallophilic macrophage morphology(MP:0008241)
    • more 1 phenotypes
  • abnormal spleen marginal zone macrophage morphology(MP:0008240)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHuman HB-EGF(DTR) cDNA, Phage P1 loxP site, Mouse Siglec1 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 19, 746-750 (2001). J. Clin Invest., 117, 2268-2278 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR/DEVELOPER (Masato Tanaka, Ph.D. and Kenji Kohno, Ph.D.) is requested.
    DepositorMasato Tanaka (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Dec 2012

    BRC mice in Publications

    Casella V, Domenjo-Vila E, Esteve-Codina A, Pedragosa M, Cebollada Rica P, Vidal E, de la Rubia I, López-Rodríguez C, Bocharov G, Argilaguet J, Meyerhans A.
    Differential kinetics of splenic CD169+ macrophage death is one underlying cause of virus infection fate regulation.
    Cell Death Dis 14(12) 838(2023) 38110339
    Chalot M, Barroca V, Devanand S, Hoffschir F, Romeo PH, Moreno SG.
    Deleterious effect of bone marrow-resident macrophages on hematopoietic stem cells in response to total body irradiation.
    Blood Adv 6(6) 1766-1779(2022) 35100346
    Borriello F, Poli V, Shrock E, Spreafico R, Liu X, Pishesha N, Carpenet C, Chou J, Di Gioia M, McGrath ME, Dillen CA, Barrett NA, Lacanfora L, Franco ME, Marongiu L, Iwakura Y, Pucci F, Kruppa MD, Ma Z, Lowman DW, Ensley HE, Nanishi E, Saito Y, O'Meara TR, Seo HS, Dhe-Paganon S, Dowling DJ, Frieman M, Elledge SJ, Levy O, Irvine DJ, Ploegh HL, Williams DL, Zanoni I.
    An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity.
    Cell 185(4) 614-629.e21(2022) 35148840
    Kim HJ, Park JH, Kim HC, Kim CW, Kang I, Lee HK.
    Blood monocyte-derived CD169+ macrophages contribute to antitumor immunity against glioblastoma.
    Nat Commun 13(1) 6211(2022) 36266311
    Millard SM, Heng O, Opperman KS, Sehgal A, Irvine KM, Kaur S, Sandrock CJ, Wu AC, Magor GW, Batoon L, Perkins AC, Noll JE, Zannettino ACW, Sester DP, Levesque JP, Hume DA, Raggatt LJ, Summers KM, Pettit AR.
    Fragmentation of tissue-resident macrophages during isolation confounds analysis of single-cell preparations from mouse hematopoietic tissues.
    Cell Rep 37(8) 110058(2021) 34818538
    Jing W, Guo X, Wang G, Bi Y, Han L, Zhu Q, Qiu C, Tanaka M, Zhao Y.
    Breast cancer cells promote CD169+ macrophage-associated immunosuppression through JAK2-mediated PD-L1 upregulation on macrophages.
    Int Immunopharmacol 78 106012(2020) 31865052
    Pirgova G, Chauveau A, MacLean AJ, Cyster JG, Arnon TI.
    Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen.
    Proc Natl Acad Sci U S A 117(22) 12295-12305(2020) 32424104
    Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC.
    Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
    J Clin Invest 129(7) 2920-2931(2019) 31039135
    Jing W, Qin F, Guo X, Sun Y, Yan C, Qiu C, Tanaka M, Shi B, Zhao Y.
    G-CSF mediates lung injury in mice with adenine-induced acute kidney injury.
    Int Immunopharmacol 63 1-8(2018) 30056257
    Li Q, Wang D, Hao S, Han X, Xia Y, Li X, Chen Y, Tanaka M, Qiu CH.
    CD169 Expressing Macrophage, a Key Subset in Mesenteric Lymph Nodes Promotes Mucosal Inflammation in Dextran Sulfate Sodium-Induced Colitis.
    Front Immunol 8 669(2017) 28694804
    Oh DS, Oh JE, Jung HE, Lee HK.
    Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection.
    Front Immunol 8 819(2017) 28751894
    Wang D, Li Q, Yang Y, Hao S, Han X, Song J, Yin Y, Li X, Tanaka M, Qiu CH.
    Macrophage Subset Expressing CD169 in Peritoneal Cavity-Regulated Mucosal Inflammation Together with Lower Levels of CCL22.
    Inflammation 40(4) 1191-1203(2017) 28466432