Strain Data Sheet

RBRC04394

Information1

Image
BRC No.RBRC04394
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Lyz2<tm1(DTR)Mtka>
Former Common nameLysM-DTR KI
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloed by Masato Tanaka, RIKEN Research Center for Allergy and Immunology in 2007.
Strain description
Colony maintenance
ReferencesJ. Immunol., 178, 5001-5009 (2007). 17404282

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lyz2lysozyme 210Lyz2<tm1(DTR)Mtka>targeted mutation 1, Masato Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)10HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAHuman HB-EGF(DTR) cDNA, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, Mouse LysM genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Biotechnol., 19, 746-750 (2001). J. Immunol., 178, 5001-5009 (2007). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Kenji Kohno,Ph.D., Masato Tanaka, Ph.D. The recipient shall obtain a prior written approval from the depositor.
DepositorMasato Tanaka (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Apr 2011
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data