Strain Data Sheet

RBRC04364

Information1

Image
BRC No.RBRC04364
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aqp4<tm1.1(GFP)Mysi>/5Rbrc
Former Common nameAquaporin-4 (AQP4) knockout mouse, AQP4 null mouse, aquaporin4 (GFP), aquaporin 4(GFP) (Acc.No. CDB0758K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentMasato Yasui, School of Medicine, Keio University/Johns Hopkins University and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology.
Strain descriptionaquaporin 4 gene GFP knock-in mice. EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04397, BALB/c background: RBRC04399), delta Pgk-neo type (#5 ES clone derived)(B6 background: RBRC04364, BALB/c mixed: RBRC04400), delta EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04401, BALB/c mixed: RBRC04402), EGFP-Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04398), delta Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04403).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesProc. Natl. Acad. Sci. USA, 116(22):11010-11019 (2019). 31097598
Mol Cell Neurosci, 56, 65-75 (2013). 23428384

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp4aquaporin 418Aqp4<tm1.1(GFP)>targeted mutation 1.1Mysi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)18EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Information2

Donor DNAphage P1 loxP sites, Jellyfish EGFP cDNA, yeast FRT (flipase recombination target) site, Mouse polyA signal, mouse Aqp4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0758K-1) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Mol Cell Neurosci, 56, 65-75 (2013). ) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

Nicosia M, Miyairi S, Beavers A, Farr GW, McGuirk PR, Pelletier MF, Valujskikh A.
Aquaporin 4 inhibition alters chemokine receptor expression and T cell trafficking.
Sci Rep 9(1) 7417(2019) 31092872

Z Heřmanová
The Role of Aquaporin 4 channels and Transient Receptor Potential Vanilloid 4 channels in astrocytic swelling.
Univerzita Karlova (2019)