RBRC04335

Information1

Image
BRC No.RBRC04335
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3-Tg(Thy1-ChR2/mCherry)9-7Sasa/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by National Institute for Basic Biology and RIKEN BioResource Center in 2010.
Strain descriptionOptogenetic transgenic mice. A transgenic construct containing ChR2 (channelrhodopsin-2)-mCherry fusion cDNA under the control of the rat thymus cell antigen, theta promoter was microinjected into B6C3F1 fertilized eggs. Expression of ChR2/mCherry has not been examined yet. Users are encouraged to test the expression pattern and feed back the data for the research community.
Colony maintenanceCarrier x Noncarrier [C57BL/6NJcl]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
References

Health Report

Examination Date / Room / Rack2011/08/29Room:3-CRack:L
2011/06/20Room:3-CRack:L
2011/04/18Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
null

Information2

Donor DNAmCherry [Discosoma sp.(class 1)], Thy1[rat genomic DNA(class 1)], COP4 (Channelrhodopsin-2)[CHLREDRAFT_182032, Chlamydomonas cDNA(class 1)],SV40 poly A [SV40 gene unit(class 2)]
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Toshikuni Sasaoka, National Institute for Basic Biology and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data