Strain Data Sheet
RBRC04335
Information1 | |
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| Image | |
| BRC No. | RBRC04335 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6;B6C3-Tg(Thy1-ChR2/mCherry)9-7Sasa/Rbrc |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by National Institute for Basic Biology and RIKEN BioResource Center in 2010. |
| Strain description | Optogenetic transgenic mice. A transgenic construct containing ChR2 (channelrhodopsin-2)-mCherry fusion cDNA under the control of the rat thymus cell antigen, theta promoter was microinjected into B6C3F1 fertilized eggs. Expression of ChR2/mCherry has not been examined yet. Users are encouraged to test the expression pattern and feed back the data for the research community. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6NJcl]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown). |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)UNChR2rat Thy1 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 poly AUN |
Information2 | |
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| Donor DNA | mCherry [Discosoma sp.(class 1)], Thy1[rat genomic DNA(class 1)], COP4 (Channelrhodopsin-2)[CHLREDRAFT_182032, Chlamydomonas cDNA(class 1)],SV40 poly A [SV40 gene unit(class 2)] |
| Research application | |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Toshikuni Sasaoka, National Institute for Basic Biology and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |