Strain Data Sheet

RBRC04333

Information1

Image
BRC No.RBRC04333
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Cyp26b1<tm2Hmd>
Former Common nameCyp26b1 flox
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2004.
Strain descriptionCyp26b1 conditional knockout mice. A loxP site is inserted into intron 4 and 3’ UTR, respectively. Conditional Cyp26b1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance
ReferencesJ. Cell Sci., 125(Pt 7):1827-36 (2012). 22366455

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cyp26b1cytochrome P450, family 26, subfamily b, polypeptide 16Cyp26b1<tm2Hmd>targeted mutation 2, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAP1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse Cyp26b1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 125(Pt 7):1827-36 (2012).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data