RBRC04293

Information1

Image
BRC No.RBRC04293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pold1<exo.uchi>
Former Common namePold1<exo.Uchi>
H-2 Haplotype
ES Cell lineNot specified (ES cell)
Background strain
Appearance
Strain developmentDeveloped by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The polyA-neo cassette was removed by crossing with Sycp1-cre mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionPold1 (polymerase (DNA directed) D400A variant mutant mice lacking 3’-5’exonuclease activity in the Pol delta. About 30% of homozygous mutant mice die with abnormally swollen thymus. Most surviving mice develop nodules on their tails. It is preferable to avoid a mating using heterozygous male across continuous generations (2 generations and up) to avoid an increasing mutation rate.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesGenome Res., 25(8)1125-1134 (2015). 26129709
PLoS One, 4, e4184 (2009). 19145245

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pold1polymerase (DNA directed), delta 1, catalytic subunit7Pold1<tm1.1Uchi>targeted mutation 1.1, Arikuni Uchimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Information2

Donor DNAP1 phage loxP site, Mouse Pold1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Dec 2015

BRC mice in Publications

No Data