RBRC04292

Information1

Image
BRC No.RBRC04292
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Pold1<tm1.uchi>
Former Common namePold1<tm1Uchi>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionPold1 (polymerase (DNA directed), delta 1, catalytic subunit) gene knockout mice. A loxP flanked polyA-neo cassette was inserted in intron 10. The exon 10 contains a D400A substitution. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J(Crlj)]
ReferencesPLoS One, 4, e4184 (2009). 19145245

Health Report

Examination Date / Room / Rack2011/05/11Room:2-3Rack:C
2011/05/09Room:2-3Rack:C
2011/04/25Room:2-3Rack:C
2011/03/08Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pold1polymerase (DNA directed), delta 1, catalytic subunit7Pold1<tm1Uchi>targeted mutation 1, Arikuni Uchimura

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal7

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), SV40 PolyA signal, P1 phage loxP, E. coli neo, mouse Pold1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data