BRC No.RBRC04222
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-KO,ChR2/GFP)459-1Sasa/Rbrc
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Strain developmentDeveloped by National Institute for Basic Biology and RIKEN BioResource Center in 2010. C57BL/6N background.
Strain descriptionOptogenetic transgenic mice. A transgenic construct containing CAG promoter, a loxP-KO (Kusabira Orange)-STOP-loxP sequence and a ChR2 (channelrhodopsin-2)-GFP fusion cDNA was microinjected into C57BL/6NCrlCrlj fertilized eggs. Expression of KO and ChR2/GFP has not been examined yet. Users are encouraged to test the expression pattern and feed back the data for the research community.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).

Health Report

Examination Date / Room / Rack2012/09/03Room:2-検Rack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
KOOrange Fluorescent Protein, Kusabira Orange (Verrillofungia concinna)UNKO


Donor DNAimmediate-esrly enhancer [K03104, CMV由来ゲノムDNA配列(クラス2)], loxP sequence [バクテリオファージ由来ゲノムDNA配列(クラス1)], GFP [クラゲ由来cDNA(クラス1)], Kusabira orange [サンゴ由来cDNA(クラス1)], betaglobin, polyA sequerce [M18818, ウサギ由来ゲノムDNA 配列(クラス1)], COP4(Channelrhodopsin-2)[CHLRDRAFT_182032, 緑藻クラミドモナス由来cDNA(クラス1),
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Toshikuni Sasaoka, National Institute for Basic Biology and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data