Strain Data Sheet

RBRC04200

Information1

Image
BRC No.RBRC04200
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cxcl12<tm2Tng>
Former Common nameCXCL12-GFP
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Takashi Nagasawa, Research Institute, Osaka Medical Center for Maternal and Child Health. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6J for 6 times, and then maintained by sibling mating.
Strain descriptionCXCL12-GFP knock-in mice. Exon 1 of the Cxcl12 gene was replaced with a GFP expression cassette.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6JJcl]
ReferencesImmunity, 19, 257-267 (2003). 12932359

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcl12chemokine (C-X-C motif) ligand 126Cxcl12<tm2Tng>targeted mutation 2, Takashi Nagasawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAJellyfish EGFP cDNA, SV40 PolyA signal, Phage P1 loxP sites, smouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Mouse Cxcl12 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
DepositorTakashi Nagasawa (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Dec 2014
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data