RBRC04111

Information1

Image
BRC No.RBRC04111
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(NDE-hsp-Pkd2,lacZ)34Hmd
Former Common nameNDE-Pkd2-IZ(34)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2000. Transgene was injected to into the pronuclei of (C57BL/6 x C3H) F1 fertilized eggs. The mice were crossed to B6/129 background strain.
Strain descriptionThese transgenic mice express Pkd2/lacZ gene on both sides of the node under the control of the node specific enhancer.
Colony maintenance
ReferencesScience, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack2011/03/08Room:2-3Rack:C
2011/01/12Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pkd2polycystic kidney disease 2UNPkd2mouse node-specific enhancer, hsp promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UN

Information2

Donor DNAマウス由来Nodalのノードエンハンサー, Pkd2cDNA, hspプロモーター, E.coli由来LacZ遺伝子, ウィルス由来IRES配列
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data