BRC No.RBRC04109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Calca<tm1Hku>
Former Common namealphaCGRP-
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Strain development
Strain descriptionThe alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT.
Colony maintenanceThe elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability.
ReferencesCirc. Res., 89, 983-990 (2001). 11717154

Health Report

Examination Date / Room / Rack2011/03/22Room:4-ARack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Calcacalcitonin/calcitonin-related polypeptide, alpha7Calca

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Calca genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ res., 89, 983-990 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorHiroki Kurihara (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P.
Effect of lentiviral vector overexpression -calcitonin gene-related peptide on titanium implant osseointegration in -CGRP-deficient mice.
Bone (2015) 26265538