Strain Data Sheet

RBRC04109

Information1

Image
BRC No.RBRC04109
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Calca<tm1Hku>
Former Common namealphaCGRP-
H-2 Haplotype
ES Cell lineSM1[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Hiroki Kurihara, Graduate School of Medicine, University of Tokyo in 2001.
Strain descriptionThe alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability.
Colony maintenanceThe elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability.
ReferencesCirc. Res., 89, 983-990 (2001). 11717154

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Calcacalcitonin/calcitonin-related polypeptide, alpha7Calca

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Calca genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ res., 89, 983-990 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorHiroki Kurihara (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Jiang Y, Yuan Y, Xiong Y, Wang B, Guo Y, Gong P, Zhang L.
Low-intensity pulsed ultrasound improves osseointegration of dental implant in mice by inducing local neuronal production of αCGRP.
Arch Oral Biol 115 104736(2020) 32371135

Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P.
Effect of lentiviral vector overexpression α-calcitonin gene-related peptide on titanium implant osseointegration in α-CGRP-deficient mice.
Bone 94 135-140(2017) 26265538

Wang B, Lin J, Zhang Q, Zhang X, Yu H, Gong P, Xiang L.
αCGRP Affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway.
Cell Transplant 28(11) 1420-1431(2019) 31426665