Strain Data Sheet

RBRC04047

Strain Information

Image
BRC No.RBRC04047
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Nfkbid<tm1Clay>
Former Common nameC57BL/6-IkBNS knockout mice
H-2 Haplotype
ES Cell lineW4 [129S6/SvEvTac]
Background strainC57BL/6NTac
Appearance
Strain developmentDeveloped by Linda K. Clayton, Dana-Farber Cancer Institute, Harvard Medical SchooI in 2001.
Strain description
Colony maintenance
References
Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor.
Fiorini E, Schmitz I, Marissen W E, Osborn S L, Touma M, Sasada T, Reche P A, Tibaldi E V, Hussey R E, Kruisbeek A M, Reinherz E L, Clayton L K
Mol. Cell., 9, 637-648 (2002). 11931770

Functional role for I kappa BNS in T cell cytokine regulation as revealed by targeted gene disruption.
Touma M, Antonini V, Kumar M, Osborn S L, Bobenchik A M, Keskin D B, Connolly J E, Grusby M J, Reinherz E L, Clayton L K
J. Immunol., 179, 1681-1692 (2007). 17641034

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nfkbidnuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, delta7Nfkbidtargeted mutation 1, Linda K Clayton
neoneomycin resistance gene (E. coli)7

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal NK cell physiology(MP:0010766)

  • abnormal thymocyte activation(MP:0003850)

  • decreased T cell proliferation(MP:0005095)

  • decreased interferon-gamma secretion(MP:0008567)

  • decreased interleukin-2 secretion(MP:0008688)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase-1 (PGK) promoter, E. coli transposon Tn5 (Neo) gene, synthetic PolyA additional signal, mouse Nfkbid genomic DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement with Dr. Ellis L. Reinherz (Dana-Farber Cancer Institute (DFCI), e-mail: Ellis_Reinherz@dfci.harvard.edu).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data