Strain Data Sheet

RBRC04029

Strain Information

Image
BRC No.RBRC04029
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6C3-Tmem50b<Gt(venusTrap_fJAV0017-B)4004-346>
Former Common nameB6C3-Tmem50b<Gt(venusTrap_fJAV0017-B)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Hiiragi, Max-Plank Institute for Molecular Biomedicine.
Strain descriptionFluorescence-based promoter gene-trap for Tmem50b (gene identity unclear) (Chr16: 91,573,058-91,573,107)
Colony maintenance
References
Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages.
Dietrich J E, Panavaite L, Gunther S, Wennekamp S, Groner A C, Pigge A, Salvenmoser S, Trono D, Hufnagel L, Hiiragi T
EMBO Rep., 16(8):1005-1021(2015). 26142281

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmem50btransmembrane protein 50B16Tmem50bFluorescence-based promoter gene-trap fJAV0017-B

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAIentivirus self-inactivating backbone (sinLV), Ientivirus self-inactivating long terminal repeat (sinLTR), adenovirus type2 major late transcript intron 1/ exon 2 splice acceptor, EMCV(Encephalomyocarditis virus) internal ribosomal entry site (IRES), jellyfish Venus (amino-acid substitution variant of GFP), SV40 nuclear localaization signal (chemically-synthesized nucleotide), Woodchuck hepatitis post-transcriptional regulatory element; Central poly-purine tract(PPT), Woodchuck hepatitis post-transcriptional regulatory element;Rev-responsive element (RRE)
    Research application
    Specific Term and Conditionsa) A recipient shall acquire a prior written consent from the Depositor using the Approval form.
    b) The use of the BIOLOGICAL RESOURCE is limited to academic research purposes only.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTakashi Hiiragi (Max Planck Institute for Moleucular Biomedicine)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data