Strain Data Sheet

RBRC04023

Strain Information

Image
BRC No.RBRC04023
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6C3-Senp2<Gt(venusTrap_fJAV0002-D)4004-321>
Former Common nameB6C3-Senp2<Gt(venusTrap_fJAV0002-D)>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Hiiragi, Max-Plank Institute for Molecular Biomedicine.
Strain descriptionFluorescence-based promoter gene-trap for Senp2 gene (Chr18: 22,035,153-22,035,623)
Colony maintenance
References
Venus trap in the mouse embryo reveals distinct molecular dynamics underlying specification of first embryonic lineages.
Dietrich J E, Panavaite L, Gunther S, Wennekamp S, Groner A C, Pigge A, Salvenmoser S, Trono D, Hufnagel L, Hiiragi T
EMBO Rep., 16(8):1005-1021(2015). 26142281

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Iresinternal ribosomal entry site (EMCV)16
Senp2SUMO/sentrin specific peptidase 216Senp2SUMO/sentrin specific peptidase 2
VenusYellow Fluorescent Protein (Aequorea victoria)16Venus
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)16WPRE
nlsSV40 large T antigen nuclear localization signal (NLS)16nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAlentivirus self-inactivating backbone (sinLV), Ientivirus self-inactivating long terminal repeat (sinLTR), adenovirus type2 major late transcript intron 1/ exon 2 splice acceptor, EMCV(Encephalomyocarditis virus) internal ribosomal entry site (IRES), jellyfish Venus (amino-acid substitution variant of GFP), SV40 nuclear localaization signal (chemically-synthesized nucleotide), Woodchuck hepatitis post-transcriptional regulatory element; Central poly-purine tract(PPT), Woodchuck hepatitis post-transcriptional regulatory element;Rev-responsive element (RRE)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditionsa) A recipient shall acquire a prior written consent from the Depositor using the Approval form.
    b) The use of the BIOLOGICAL RESOURCE is limited to academic research purposes only.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorTakashi Hiiragi (Max Planck Institute for Moleucular Biomedicine)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data