BRC No.RBRC04021
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dnase2a<tm1Osa> Ifnar1<tm1Agt>
Former Common nameDNaseII / IFN-IR double knock out
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Strain developmentDeveloped by Kohki Kawane and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2004. C57BL/6 background (over N8 generations).
Strain descriptionDNaseII/IFN-IR double knockout mice. Double homozygous mutant mice develop polyarthritis as age.
Colony maintenanceDnase2a: Heterozygote x Wild-type; Ifnar1: Homozygote x Homozygote
ReferencesScience, 264, 1918-1921 (1994). 8009221
Nat. Immunol., 6, 49-56 (2005). 15568025
Nature, 443, 998-1002 (2006). 17066036

Health Report

Examination Date / Room / Rack2020/06/15Room:3-ARack:G


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ifnar1interferon (alpha and beta) receptor 116Ifnar1<tm1Agt>targeted mutation 1, Michel Aguet

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)16herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dnase2adeoxyribonuclease II alpha8Dnase2a<tm1Osa>targeted mutation 1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNABacteriophage P1 loxP sequence, Mouse Pgk1 genomic DNA, E. coli Neomycin resistance gene, mouse Dnase2a genomic DNA, Herpes simplex virus Thymidine kinase promoter (HSV-tk promoter), Polyoma virus genomic DNA, mouse Ifnar1 genomic DNA [update March 10, 2014]
Research applicationCre/loxP system
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 443, 998-1002 (2006). Nat. Immunol., 6, 49-56 (2005).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-
Mouse of the Month Aug 2012

BRC mice in Publications

No Data