Strain Data Sheet


Strain Information

BRC No.RBRC04021
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dnase2a<tm1Osa> Ifnar1<tm1Agt>
Former Common nameDNaseII / IFN-IR double knock out
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Strain developmentDeveloped by Kohki Kawane and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2004. C57BL/6 background (over N8 generations).
Strain descriptionDNaseII/IFN-IR double knockout mice. Double homozygous mutant mice develop polyarthritis as age.
Colony maintenanceDnase2a: Heterozygote x Wild-type; Ifnar1: Homozygote x Homozygote
ReferencesNature, 443, 998-1002 (2006). 17066036
Nat. Immunol., 6, 49-56 (2005). 15568025
Science, 264, 1918-1921 (1994). 8009221

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ifnar1interferon (alpha and beta) receptor 116Ifnar1<tm1Agt>targeted mutation 1, Michel Aguet

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)16herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnase2adeoxyribonuclease II alpha8Dnase2a<tm1Osa>targeted mutation 1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNABacteriophage P1 loxP sequence, Mouse Pgk1 genomic DNA, E. coli Neomycin resistance gene, mouse Dnase2a genomic DNA, Herpes simplex virus Thymidine kinase promoter (HSV-tk promoter), Polyoma virus genomic DNA, mouse Ifnar1 genomic DNA [update March 10, 2014]
Research applicationCre/loxP system
Immunology and Inflammation Research
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 443, 998-1002 (2006). Nat. Immunol., 6, 49-56 (2005).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-
Mouse of the Month Aug 2012

BRC mice in Publications

Yoshikawa R, Sakabe S, Urata S, Yasuda J.
Species-Specific Pathogenicity of Severe Fever with Thrombocytopenia Syndrome Virus Is Determined by Anti-STAT2 Activity of NSs.
J Virol 93(10) (2019) 30814285