BRC No.RBRC04020
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Dnase2a<tm2.1Osa>
Former Common nameDNaseII conditional knock out
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6
Strain development
Strain description
Colony maintenance
ReferencesNature, 443, 998-1002 (2006). 17066036

Health Report

Examination Date / Room / Rack2017/05/29Room:4-BRack:B


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dnase2adeoxyribonuclease II alpha8Dnase2a<tm2.1Osa>targeted mutation 2.1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP


Donor DNAphage P1 loxP sites, mouse Dnase2a genomic DNA [update May 20, 2015]
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 443, 998-1002 (2006).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose. The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-
Mouse of the Month Aug 2012
Genetic Background

BRC mice in Publications

Chan MP, Onji M, Fukui R, Kawane K, Shibata T, Saitoh S, Ohto U, Shimizu T, Barber GN, Miyake K.
DNase II-dependent DNA digestion is required for DNA sensing by TLR9.
Nat Commun 6 5853(2015) 25600358