RBRC03941

Information1

Image
BRC No.RBRC03941
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Sqstm1<tm2(GFP)Keta>/Rbrc
Former Common name
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Cell Sci., 128(23), 4453-4461 (2015). 26483381

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sqstm1sequestosome 111Sqstm1<tm2(GFP)Keta>targeted mutation 2, Keiji Tanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA11

Information2

Donor DNAmouse Sqstm1(exon 3-8) cDNA, Jellyfish GFP cDNA, SV40 polyA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Sqstm1 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Keiji Tanaka, Tokyo Metropolitan Institute of Medical Science, Dr. Masaaki Komatsu, Niigata University and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature is requested. J. Cell Sci., 128(23), 4453-4461 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit oraganization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Eino A, Kageyama S, Uemura T, Annoh H, Saito T, Narita I, Waguri S, Komatsu M.
Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.
J. Cell. Sci. 128(23) 4453-61(2015) 26483381