Strain Information | |
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Image | |
BRC No. | RBRC03941 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Sqstm1<tm2(GFP)Keta>/Rbrc |
Former Common name | |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6NCrlCrlj |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. Eino A, Kageyama S, Uemura T, Annoh H, Saito T, Narita I, Waguri S, Komatsu M J. Cell Sci., 128(23), 4453-4461 (2015). 26483381 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
SV40 polyA | 11 | more 1 Diseases | |||||
GFP | Green Fluorescent Protein (Aequorea victoria) | 11 | more 1 Diseases | ||||
Sqstm1 | sequestosome 1 | 11 | Sqstm1 | targeted mutation 2, Keiji Tanaka | more 1 Diseases | ||
neo | neomycin resistance gene (E. coli) | 11 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) | more 1 Diseases |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Sqstm1(exon 3-8) cDNA, Jellyfish GFP cDNA, SV40 polyA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Sqstm1 genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Keiji Tanaka, Tokyo Metropolitan Institute of Medical Science, Dr. Masaaki Komatsu, Niigata University and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature is requested. J. Cell Sci., 128(23), 4453-4461 (2015). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit oraganization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Eino A, Kageyama S, Uemura T, Annoh H, Saito T, Narita I, Waguri S, Komatsu M. Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells. J Cell Sci 128(23) 4453-61(2015) 26483381 |