RBRC03801

Information1

Image
BRC No.RBRC03801
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(Vil1-cre)2Utr/Rbrc
Former Common nameVillin-NLSCre
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background.
Strain descriptionCre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse villin 1 promoter fragment (intestinal stem cells specific promoter region) derived from the BAC library was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community.
Colony maintenanceCarrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
References

Health Report

Examination Date / Room / Rack2020/06/01Room:3-ARack:D
2020/02/17Room:3-ARack:D
2019/12/16Room:3-ARack:D
2019/10/21Room:3-ARack:D
2019/08/26Room:3-ARack:D
2019/08/26Room:3-ARack:D
2019/06/17Room:3-ARack:D
2019/06/17Room:3-ARack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnlsmouse Villin gene promoter (intestinal stem cells specific promoter region)

Information2

Donor DNAP1 phage Cre gene, yeast FRT (flipase recombination target) site, Mouse Villin gene promoter (intestinal stem cells specific promoter region), Phage P1 loxP derived from pBACe3.6 vector, Simian virus 40 Large T antigen nuclear localization signal (NLS), poly A
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Jul 2013

BRC mice in Publications

No Data