RBRC03743

Information1

Image
BRC No.RBRC03743
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common nameB6D2F1-Tg(CAG/su9-DsRed2,Acr3-EGFP), RBGS002Osb (Nov. 2011), RBGS-002
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with Acr3-EGFP and CAG/su9-DsRed2 transgene constructs into fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain descriptionTrangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9).
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6D2F1]
ReferencesExp. Anim., 59:105-107(2010). 20224175
Animal Experimentation, 59(1), (2009).

Health Report

Examination Date / Room / Rack2020/06/23Room:3-BRack:C
2020/06/01Room:3-BRack:C
2020/02/17Room:3-BRack:C
2019/12/16Room:3-BRack:C
2019/10/21Room:3-BRack:C
2019/08/26Room:3-BRack:C
2019/06/17Room:3-BRack:C
2019/04/15Room:3-BRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFPacrosin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
su9mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]UNsu9CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)], Discosoma sp. DsRed2 cDNA, acrosin promoter, acrosin localization signal, jellyfish EGFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 59:105-107(2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Category I MTA: FP license of TBUSA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

Hino T, Muro Y, Tamura-Nakano M, Okabe M, Tateno H, Yanagimachi R.
The Behavior and Acrosomal Status of Mouse Spermatozoa In Vitro, and Within the Oviduct During Fertilization After Natural Mating.
Biol. Reprod. (2016) 27417908

L?ck JC, Puchkov D, Ullrich F, Jentsch TJ.
LRRC8/VRAC anion channels are required for late stages of spermatid development in mice.
J. Biol. Chem. (2018) 29880644

Luo SM, Ge ZJ, Wang ZW, Jiang ZZ, Wang ZB, Ouyang YC, Hou Y, Schatten H, Sun QY.
Unique insights into maternal mitochondrial inheritance in mice.
Proc. Natl. Acad. Sci. U.S.A. 110(32) 13038-43(2013) 23878233