Strain Data Sheet

RBRC03733

Information1

Image
BRC No.RBRC03733
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt3b<tm5.1Enl>
Former Common nameDnmt3b<tm5.1Enl>; Dnmt3b conditional KO, Dnmt3b-2lox
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Masaki Okano and En Li, Massachusetts General Hospital in 2001. J1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6.
Strain descriptionDnmt3b floxed mice. Exon 19 of the Enmt3b was flanked by loxP sites. Conditional Dnmt3a deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Dnmt3a knockout mice (RBRC03730), Dnmt3a floxed mice (RBRC03731), Dnmt3b knockout mice (RBRC03732).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesJ. Biol. Chem., 280, 17986-17991 (2005). 15757890

Health Report

Examination Date / Room / Rack2022/06/13Room:3-2Rack:A
2022/03/14Room:3-2Rack:A
2021/12/14Room:3-2Rack:A
2021/09/13Room:3-2Rack:A
2021/06/14Room:3-2Rack:A
2021/03/29Room:3-2Rack:A
2021/01/25Room:3-2Rack:A
2021/01/05Room:3-2Rack:A
2020/10/05Room:3-2Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnmt3bDNA methyltransferase 3B2Dnmt3b<tm5.1Enl>targeted mutation 5.1, En Li

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Information2

Donor DNAPhage P1 LoxP sites, mouse Dnmt3b genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsA. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature J Biol Chem. 2005 280:17986-91 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.
DepositorMasaki Okano (RIKEN CDB/ MGH)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Matsuzaki H, Okamura E, Takahashi T, Ushiki A, Nakamura T, Nakano T, Hata K, Fukamizu A, Tanimoto K.
De novo DNA methylation through the 5'-segment of the H19 ICR maintains its imprint during early embryogenesis.
Development 142(22) 3833-44(2015) 26417043