Strain Data Sheet



BRC No.RBRC03732
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt3b<tm1Enl>
Former Common nameDnmt3b<tm1Enl>; Dnmt3b null KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Strain developmentDeveloped by Masaki Okano and En Li, Massachusetts General Hospital in 1999. J1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6.
Strain descriptionDnmt3b knockout mice. A 2.4 kb region encoding the PC and EV motifs was replaced with an IRES-beta-geo cassette. Homozygous mutant mice are embryonic lethal (E14.5-16.5). Dnmt3a knockout mice (RBRC03730), Dnmt3a floxed mice (RBRC03731), Dnmt3b floxed mice (RBRC03733).
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesCell, 99, 247-257 (1999). 10555141

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dnmt3bDNA methyltransferase 3B2Dnmt3b<tm1Enl>targeted mutation 1, En Li

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)2En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal2


Donor DNAmouse En-2 splice acceptor, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, E. coli neomycin registance gene, SV40 poly A signal sequence, mouse Dnmt3b genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsA. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA ( B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Cell 1999 99:247-57 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.
DepositorMasaki Okano (RIKEN CDB/ MGH)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data