BRC No.RBRC03730
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Dnmt3a<tm1Enl>
Former Common nameDnmt3a<tm1Enl>; Dnmt3a null KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Strain developmentDeveloped by Masaki Okano and En Li, Massachusetts General Hospital in 1999. J1 ES cells were used to generate the mutant mice. The mutant mice were crossed to C57BL/6.
Strain descriptionDnmt3a knockout mice. A 0.3 kb region containing exons of Dnmt3a encoding PC and ENV motif was replaced with an IRES-beta-geo cassette. Homozygous mutant mice appear normal at birth, but most mutant mice become runted and die at about 4 week of age. Dnmt3a floxed mice (RBRC03731), Dnmt3b knockout mice (RBRC03732), Dnmt3b floxed mice (RBRC03733).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesCell, 99, 247-257 (1999). 10555141

Health Report

Examination Date / Room / Rack2012/11/26Room:4-ARack:I


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Dnmt3aDNA (cytosine-5)-methyltransferase 3A isoform 112Dnmt3a<tm1Enl>targeted mutation 1, En Li

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12


Donor DNAmouse En-2 splice acceptor, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, E. coli neomycin registance gene, SV40 poly A signal sequence, mouse Dnmt3a genomic DNA
Research application
Specific Term and ConditionsA. A RECIPIENT must obtain a written permission of the DEPOSITOR. RECIPIENT must send a copy of the executed MTA between RECIPIENT and RIKEN BRC to: Massachusetts General Hospital c/o Partners Innovation, Attn: TAG/MGH MTA 2020A005592, 399 Revolution Drive/Ste 950E, Somerville,MA 02245, USA (phsmta@partners.org). B. Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic ("Name") of MGH or any of its affiliates, or any of its or their respective directors, trustees, officers, appointees, employees, staff, representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. C. In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature Cell 1999 99:247-57 as designated by the DEPOSITOR is required. D. The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. E. The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. F. Recipients shall assume all liability for their use, storage, handling and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposal of the BIOLOGICAL RESOURCE by Recipient.
DepositorMasaki Okano (RIKEN CDB/ MGH)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Please use the MATERIAL TRANSFER AGREEMENT for Distribution for non-profit or for profit (English only).
Breeding characters

BRC mice in Publications

No Data