RBRC03709

Information1

Image
BRC No.RBRC03709
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Disc1<tm1Kozo>
Former Common nameDISC1 (delta 2-3), DISC1 KO, Disc1(-) (Acc.No. CDB0583K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Keisuke Kuroda, Kozo Kaibuchi, Nagoya University, Graduate School of Medicine in 2008. A loxP/PGK-Neo-pA/loxP cassette was transferred into TT2 ES cells to replace most of exon 2 and all of exon 3 of Disc1 gene. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionDisc1 knockout mice. Most of exon 2 and all of exon 3 of Disc1 gene were replaced with a loxP/PGK-Neo-pA/loxP cassette. Homozygous mutant mice are viable and fertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesHum. Mol. Genet., 20, 4666-4683 (2011). 21903668

Health Report

Examination Date / Room / Rack2014/11/17Room:4-ARack:I
2014/09/16Room:4-ARack:I
2014/07/14Room:4-ARack:I
2010/07/05Room:2-3Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Disc1disrupted in schizophrenia 18Disc1<tm1Kozo>targeted mutation 1, Kozo Kaibuchi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neomycin resistance gene, Mouse phosphoglycerate kinase Poly A signal, mouse Disc1 genome DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 20, 4666-4683 (2011).(1) The RECIPIENT shall obtain a prior agreement from Dr. Kouzo Kaibuchi (kaibuchi@med.nagoya-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0583K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未定) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Onishi T, Sakamoto H, Namiki S, Hirose K.
The Altered Supramolecular Structure of Dopamine D2 Receptors in Disc1-deficient Mice.
Sci Rep 8(1) 1692(2018) 29374282