Strain Data Sheet


Strain Information

BRC No.RBRC03674
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Gad1<tm1.1Tama>
Former Common nameGAD67-GFP dNeo KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Strain developmentDeveloped by Yuchio Yanagawa, Graduate School of Medicine, Gumma University. ICR mixed background.
Strain descriptionAn EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are neonatal lethal due to cleft palate. A loxP flanked neo was removed by crossing with cre mice.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]
ReferencesJ. Comp. Neurol., 467, 60-79 (2003). 14574680

Health Report

Examination Date / Room / Rack2024/05/27Room:4-BRack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gad1glutamate decarboxylase 12Gad1<tm1.1Tama>targeted mutation 1.1, Nobuaki Tamamaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

Donor DNAJellyfish green fluorescent protein cDNA, phage P1loxP site, mouse Gad1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp Neurol., 467, 60-79 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without DEPOSITOT's permission. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYuchio Yanagawa (Gunma University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Jan 2014

BRC mice in Publications

Jahan MS, Ito T, Ichihashi S, Masuda T, Bhuiyan MER, Takahashi I, Takamatsu H, Kumanogoh A, Tsuzuki T, Negishi T, Yukawa K.
PlexinA1 deficiency in BALB/cAJ mice leads to excessive self-grooming and reduced prepulse inhibition.
IBRO Rep 9 276-289(2020) 33163687

Saito K, Koike T, Kawashima F, Kurata H, Shibuya T, Satoh T, Hata Y, Yamada H, Mori T.
Identification of NeuN immunopositive cells in the adult mouse subventricular zone.
J Comp Neurol 526(12) 1927-1942(2018) 29752725

Carstens KE, Phillips ML, Pozzo-Miller L, Weinberg RJ, Dudek SM.
Perineuronal Nets Suppress Plasticity of Excitatory Synapses on CA2 Pyramidal Neurons.
J Neurosci 36(23) 6312-20(2016) 27277807

Fu X, Teboul E, Weiss GL, Antonoudiou P, Borkar CD, Fadok JP, Maguire J, Tasker JG.
Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice.
Nat Commun 13(1) 1290(2022) 35277502