Strain Data Sheet

RBRC03674

Strain Information

Image
BRC No.RBRC03674
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Gad1<tm1.1Tama>
Former Common nameGAD67-GFP dNeo KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Graduate School of Medicine, Gumma University. ICR mixed background.
Strain descriptionAn EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are neonatal lethal due to cleft palate. A loxP flanked neo was removed by crossing with cre mice.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]
References
Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
Tamamaki N, Yanagawa Y, Tomioka R, Miyazaki J, Obata K, Kaneko T
J. Comp. Neurol., 467, 60-79 (2003). 14574680

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:D
2024/05/27Room:4-BRack:D
2024/02/26Room:4-BRack:D
2023/11/27Room:4-BRack:D
2023/08/28Room:4-BRack:D
2023/05/29Room:4-BRack:D
2023/04/10Room:4-BRack:D
2023/02/27Room:4-BRack:D

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP
  • developmental and epileptic encephalopathy 89(MedGEN)
    • Gad1glutamate decarboxylase 12Gad1targeted mutation 1.1, Nobuaki Tamamaki
  • developmental and epileptic encephalopathy 89(MedGEN)
    • loxPphage P1 loxP2loxP
  • developmental and epileptic encephalopathy 89(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal GABAergic neuron physiology(MP:0011987)

  • abnormal respiratory function(MP:0002327)

  • abnormal respiratory system development(MP:0003115)

  • abnormal synaptic neurotransmitter level(MP:0002204)

  • cleft palate(MP:0000111)
    • more 3 phenotypes
  • decreased neurotransmitter release(MP:0003990)

  • nervous system phenotype(MP:0003631)

  • submucous cleft palate(MP:0011615)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish green fluorescent protein cDNA, phage P1loxP site, mouse Gad1 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp Neurol., 467, 60-79 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without DEPOSITOT's permission. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorYuchio Yanagawa (Gunma University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jan 2014

    BRC mice in Publications

    Jahan MS, Ito T, Ichihashi S, Masuda T, Bhuiyan MER, Takahashi I, Takamatsu H, Kumanogoh A, Tsuzuki T, Negishi T, Yukawa K.
    PlexinA1 deficiency in BALB/cAJ mice leads to excessive self-grooming and reduced prepulse inhibition.
    IBRO Rep 9 276-289(2020) 33163687
    Minami-Ogawa Y, Kiyokage E, Yamanishi H, Horie S, Ichikawa S, Toida K.
    Structural Basis for Histaminergic Regulation of Neural Circuits in the Mouse Olfactory Bulb.
    J Comp Neurol 532(10) e25671(2024) 39387358
    Saito K, Koike T, Kawashima F, Kurata H, Shibuya T, Satoh T, Hata Y, Yamada H, Mori T.
    Identification of NeuN immunopositive cells in the adult mouse subventricular zone.
    J Comp Neurol 526(12) 1927-1942(2018) 29752725
    Carstens KE, Phillips ML, Pozzo-Miller L, Weinberg RJ, Dudek SM.
    Perineuronal Nets Suppress Plasticity of Excitatory Synapses on CA2 Pyramidal Neurons.
    J Neurosci 36(23) 6312-20(2016) 27277807
    Fu X, Teboul E, Weiss GL, Antonoudiou P, Borkar CD, Fadok JP, Maguire J, Tasker JG.
    Gq neuromodulation of BLA parvalbumin interneurons induces burst firing and mediates fear-associated network and behavioral state transition in mice.
    Nat Commun 13(1) 1290(2022) 35277502