RBRC03674

Information1

Image
BRC No.RBRC03674
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR.Cg-Gad1<tm1.1Tama>
Former Common nameGAD67-GFP dNeo KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yuchio Yanagawa, Graduate School of Medicine, Gumma University. ICR mixed background.
Strain descriptionAn EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are neonatal lethal due to cleft palate. A loxP flanked neo was removed by crossing with cre mice.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]
ReferencesJ. Comp. Neurol., 467, 60-79 (2003). 14574680

Health Report

Examination Date / Room / Rack2020/03/30Room:4-BRack:D
2020/01/27Room:4-BRack:D
2019/11/25Room:4-BRack:D
2019/09/30Room:4-BRack:D
2019/07/29Room:4-BRack:D
2019/05/27Room:4-BRack:D
2019/03/25Room:4-BRack:D
2019/01/28Room:4-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gad1glutamate decarboxylase 12Gad1<tm1.1Tama>targeted mutation 1.1, Nobuaki Tamamaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)2EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP2loxP

Information2

Donor DNAJellyfish green fluorescent protein cDNA, phage P1loxP site, mouse Gad1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp Neurol., 467, 60-79 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without DEPOSITOT's permission. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYuchio Yanagawa (Gunma University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Mouse of the Month Jan 2014

BRC mice in Publications

Saito K, Koike T, Kawashima F, Kurata H, Shibuya T, Satoh T, Hata Y, Yamada H, Mori T.
Identification of NeuN immunopositive cells in the adult mouse subventricular zone.
J. Comp. Neurol. (2018) 29752725

Carstens KE, Phillips ML, Pozzo-Miller L, Weinberg RJ, Dudek SM.
Perineuronal Nets Suppress Plasticity of Excitatory Synapses on CA2 Pyramidal Neurons.
J. Neurosci. 36(23) 6312-20. (2016) 27277807