Strain Data Sheet

RBRC03626

Strain Information

Image
BRC No.RBRC03626
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(EF1A-BCL2)1Ymat
Former Common nameBCL2 Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhisa Matsui, Institute of Development, Aging and Cancer, Tohoku University.
Strain descriptionThese transgenic mice express human BCL2 under the control of the human EF1A promoter. Male infertile.
Colony maintenanceCarrier x Noncarrier
References
Inhibition of testicular germ cell apoptosis and differentiation in mice misexpressing Bcl-2 in spermatogonia.
Furuchi T, Masuko K, Nishimune Y, Obinata M, Matsui Y
Development, 122, 1703-1709 (1996). 8674410

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
BCL2B-cell leukemia/lymphoma 2 (human)UNBCL2human elongation factor 1 alpha (EF-1 alpha) promoter
GHGrowth hormone polyA (Bovine)UNGH

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal spermatocyte morphology(MP:0006379)

  • abnormal spermatogenesis(MP:0001156)

  • abnormal testis size(MP:0004849)

  • abnormal testis weight(MP:0004850)

  • enlarged testis(MP:0001148)
    • more 2 phenotypes
  • reduced female fertility(MP:0001923)

  • reduced male fertility(MP:0001922)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEF1a promoter (human), bcl-2 cDNA (human), PolyA signal (bovine)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 122, 1703-1709 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYasuhisa Matsui (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data