RBRC03558

Information1

Image
BRC No.RBRC03558
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129.Cg-Pax7<tm1Pgr>
Former Common namePax129, 129-Pax7 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Peter Gruss, Max Planck Institute for Biophysical Chemistry. The mutant mice were backcrossed 129 over 20 times by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.
Strain descriptionPax7 gene knockout mice. The first exon of the paired box was replaced with a neo cassette (RBRC03558) and lacZ-neo cassette (RBRC03559), respectively. In the C57BL/6 background, most homozygous mutant mice die within 3 weeks after birth (survival rate is below 10%). On the other hand, in the 129 background (RBRC03558 and RBRC03559), approximately 50% of mutant mice die within 3 weeks after birth (details unknown). Heterozygous mutant mice for lacZ repoter Pax7<tm2Pgr> (RBRC03559) show negative X-gal staining in the muscle satellite cells.
Colony maintenanceHeterozygote x Wild-type [or Crossing to 129<+Ter>/SvJcl]
ReferencesCell, 102, 777-786 (2000). 11030621
Development, 122, 831-838 (1996). 8631261

Health Report

Examination Date / Room / Rack2020/03/30Room:4-CRack:K
2020/01/27Room:4-CRack:K
2019/11/25Room:4-CRack:K
2019/09/30Room:4-CRack:K
2019/07/29Room:4-CRack:K
2019/05/27Room:4-CRack:K
2019/03/25Room:4-CRack:K
2019/01/28Room:4-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pax7paired box gene 74Pax7<tm1Pgr>targeted mutation 1, Peter Gruss

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (Klebsiella pneumoniae)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Myod1 genomic DNA, Klebsiella pneumoniae neomycin resistance gene, mouse phosphoglycerate kinase promoter (PGK promoter), bovine growth hormone polyadenylation signal
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Pax7tm1Pgr allele (Peter Gruss, Max Planck Institute for Biophysical Chemistry). (https://www.oist.jp/peter-gruss)
DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityLive mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
MATERIAL TRANSFER AGREEMENT (English)

BRC mice in Publications

No Data