Strain Data Sheet

RBRC03556

Strain Information

Image
BRC No.RBRC03556
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129-Dmd<tm1Mok>
Former Common nameMdx52, B6-Dmd KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, Institute of Medical Science, University of Tokyo. CCE/EK.CCE ES cells were used. The mutant mice were backcrossed to C57BL/6 by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.
Strain descriptiondystrophin (Dmd) gene knockout mice. The exon 52 was replaced with a neo cassette. Mutant mice show hypertrophic skeletal muscles. Homozygous female and hemizygous male mutants are viable and fertile. The mice are difficult to start breeding at 8-10 weeks (12-16 weeks is better).
Colony maintenanceHomozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 238, 492-497 (1997). 9299538

Health Report

Examination Date / Room / Rack2023/04/10Room:3-BRack:B
2023/01/10Room:3-BRack:B
2022/10/11Room:3-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dmddystrophin, muscular dystrophyXDmd<tm1Mok>targeted mutation 1, Motoya Katsuki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Klebsiella pneumoniae)Xherpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), Klebsiella pneumoniae neomycin resistance gene, mouse Dmd genomic DNA
Research applicationMouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 238, 492-497 (1997).
DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Apr 2014

BRC mice in Publications

Motohashi N, Minegishi K, Aoki Y.
Inherited myogenic abilities in muscle precursor cells defined by the mitochondrial complex I-encoding protein.
Cell Death Dis 14(10) 689(2023) 37857600