Strain Data Sheet

RBRC03551

Strain Information

Image
BRC No.RBRC03551
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Abcd1<tm1Ymd>
Former Common name
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yamada, Faculty of Medicine, Kyushu University. CCE/EK.CCE ES cells were used to generate the mutant mice. C57BL/6, 129 and DBA/2 mixed (RBRC03551), C57BL/6 congenic (RBRC03552).
Strain descriptionALDP (Abcd1) gene knockout mice. The 3’ part of exon 1 was replaced with a neo cassette.
Colony maintenance
References
Adrenoleukodystrophy protein-deficient mice represent abnormality of very long chain fatty acid metabolism.
T Kobayashi, N Shinnoh, A Kondo, T Yamada
Biochem. Biophys. Res. Commun., 232, 631-636 (1997). 9126326

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Abcd1
MGI:1349215		ATP-binding cassette, sub-family D member 1XAbcd1<tm1Ymd>
MGI:2429945		targeted mutation 1, Takeshi Yamada
  • adrenoleukodystrophy(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)X herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal lipid homeostasis (MP:0002118)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, mouse Abcd1 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem Biophys Res Commun, 232, 631-636 (1997).
    DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Fujitani N, Akashi T, Saito M, Morita M, So T, Oka K.
    Increased neurotoxicity of high-density lipoprotein secreted from murine reactive astrocytes deficient in a peroxisomal very-long-chain fatty acid transporter Abcd1.
    J Inherit Metab Dis (2023) 38146202