Strain Data Sheet

RBRC03519

Strain Information

Image
BRC No.RBRC03519
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2;129P2-Cthrc1<tm1Hssk>/Rbrc
Former Common nameCthrc1(lacZ) (Acc.No. CDB0502K)
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Sasaki, RIKEN Center for Developmental Biology. A lacZ and PGK-neo cassette was transferred into E14TG2a ES cells to replace the exon 1 of Cthrc1 gene.
Strain descriptionCthrc1 gene knockout mice. Cthrc1 is a Wnt signaling component that selectively activates the PCP pathway.
Colony maintenance
ReferencesDev. Cell., 15, 23-36 (2008). 18606138

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cthrc1collagen triple helix repeat containing 115Cthrc1<tm1Hssk>targeted mutation 1, Hiroshi Ssasaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin 3' end15HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence15lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15

Ordering Information

Donor DNAPhage P1 lox71 site and loxP site, E. coli lacZ, rabbit beta-globin 3' end, yeast FRT (flipase recombination target) sites, mouse PGK promoter and PGK 3' end, E. coli neo, mouse Cthrc1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0502K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Cell. 2008 15(1):23-36.) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Li J, Wang Y, Ma M, Jiang S, Zhang X, Zhang Y, Yang X, Xu C, Tian G, Li Q, Wang Y, Zhu L, Nie H, Feng M, Xia Q, Gu J, Xu Q, Zhang Z.
Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-β signaling.
EBioMedicine 40 43-55(2019) 30639416