RBRC03518

Information1

Image
BRC No.RBRC03518
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameDraxin(lacZ)(Acc.No. CDB0484K)
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesScience, 323, 388-393 (2009). 19150847

Health Report

Examination Date / Room / Rack2017/06/05Room:3-2Rack:C
2017/04/03Room:3-2Rack:C
2017/02/06Room:3-2Rack:C
2016/12/05Room:3-2Rack:C
2016/10/03Room:3-2Rack:C
2016/08/08Room:3-2Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Draxindorsal inhibitory axon guidance protein4Draxin<tm1Htnk>targeted mutation 1, Hideaki Tanaka

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence4lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 lox71 site, E. coli lacZ, rabbit beta-globin pA (genomic DNA), yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase (Pgk1) promoter, E. coli neo, mouse phosphoglycerate kinase (Pgk1) pA, phage P1 loxP site, mouse Draxin genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Yohei Shinmyo (shinmyo@med.kanazawa-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0484K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Science. 2009 323(5912):388-93.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data