Strain Data Sheet

RBRC03518

Strain Information

Image
BRC No.RBRC03518
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-2610109H07Rik<tm1Htnk>/Rbrc
Former Common nameDraxin(lacZ)(Acc.No. CDB0484K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Draxin, a repulsive guidance protein for spinal cord and forebrain commissures.
Islam S M, Shinmyo Y, Okafuji T, Su Y, Naser I B, Ahmed G, Zhang S, Chen S, Ohta K, Kiyonari H, Abe T, Tanaka S, Nishinakamura R, Terashima T, Kitamura T, Tanaka H
Science, 323, 388-393 (2009). 19150847

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Draxindorsal inhibitory axon guidance protein4Draxintargeted mutation 1, Hideaki Tanaka
Frtyeast FRT (flippase recombination target) site4Frt
Frtyeast FRT (flippase recombination target) site4Frt
lacZbeta-galactosidase (E. coli)4
lox71mutant lox sequence4lox71
loxPphage P1 loxP4loxP
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal spinal cord interneuron morphology(MP:0004100)

  • absent anterior commissure(MP:0008227)

  • absent corpus callosum(MP:0002196)

  • absent hippocampal commissure(MP:0008223)

  • decreased anterior commissure size(MP:0008226)
    • more 2 phenotypes
  • decreased corpus callosum size(MP:0000781)

  • decreased hippocampal commissure size(MP:0008222)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 lox71 site, E. coli lacZ, rabbit beta-globin pA (genomic DNA), yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase (Pgk1) promoter, E. coli neo, mouse phosphoglycerate kinase (Pgk1) pA, phage P1 loxP site, mouse Draxin genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Yohei Shinmyo (shinmyo@med.kanazawa-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0484K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Science. 2009 323(5912):388-93.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data