RBRC03515

Information1

Image
BRC No.RBRC03515
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aire<tm2Mmat>/Rbrc
Former Common nameAire(GFP) (Acc.No. CDB0479K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Mitsuru Matsumoto, Institute for Enzyme Research, University of Tokushima. TT2 ES cells were used.
Strain descriptionAire GFP knock-in mice. A GFP was inserted in the Aire gene locus between exon 1 and 2.
Colony maintenance
ReferencesJ. Exp. Med., 205, 2827-2838 (2008). 19015306

Health Report

Examination Date / Room / Rack2014/10/29Room:3-CRack:E
2014/10/28Room:3-CRack:E
2014/10/20Room:3-CRack:E
2014/08/18Room:3-CRack:E
2014/07/30Room:3-CRack:E
2014/06/16Room:3-CRack:E
2014/04/30Room:3-CRack:E
2014/04/21Room:3-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Aireautoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)10Aire<tm2Mmat>targeted mutation 2, Mitsuru Matsumoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)10

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neo, jellyfish GFP cDNA, mouse Aire genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Mitsuru Matsumoto (mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0479K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2008 205(12):2827-38.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data