RBRC03513

Information1

Image
BRC No.RBRC03513
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Isx<tm1Sse>/Rbrc
Former Common nameIsx(lacZ) (Acc.No. CDB0475K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Susumu Seino, Kobe University Graduate School of Medicine. TT2 ES cells were used to generate the mutant mice.
Strain descriptionIsx gene knockout mice. Exon1 was replaced with a lacZ-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesJ. Biol. Chem., 283, 4905-4911 (2008). 18093975

Health Report

Examination Date / Room / Rack2015/04/20Room:3-CRack:F
2015/02/16Room:3-CRack:F
2014/12/15Room:3-CRack:F
2014/10/20Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Isxintestine specific homeobox8Isx<tm1Sse>targeted mutation 1, Susumu Seino

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA8HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)8

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence8lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 lox71, E. coli LacZ, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Phage P1 loxP, rabbit beta-globin polyA, mouse Isx genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Susumu Seino (seino@med.kobe-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0475K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Biol Chem. 2008 283(8):4905-11.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data