Strain Data Sheet

RBRC03513

Strain Information

Image
BRC No.RBRC03513
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Isx<tm1Sse>/Rbrc
Former Common nameIsx(lacZ) (Acc.No. CDB0475K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Susumu Seino, Kobe University Graduate School of Medicine. TT2 ES cells were used to generate the mutant mice.
Strain descriptionIsx gene knockout mice. Exon1 was replaced with a lacZ-neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References
Isx participates in the maintenance of vitamin A metabolism by regulation of beta-carotene 15,15'-monooxygenase (Bcmo1) expression.
Seino Y, Miki T, Kiyonari H, Abe T, Fujimoto W, Kimura K, Takeuchi A, Takahashi Y, Oiso Y, Iwanaga T, Seino S
J. Biol. Chem., 283, 4905-4911 (2008). 18093975

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site8Frt
Frtyeast FRT (flippase recombination target) site8Frt
HBB2rabbit beta-globin polyA8HBB2
Isxintestine specific homeobox8Isxtargeted mutation 1, Susumu Seino
lacZbeta-galactosidase (E. coli)8
lox71mutant lox sequence8lox71
loxPphage P1 loxP8loxP
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 lox71, E. coli LacZ, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Phage P1 loxP, rabbit beta-globin polyA, mouse Isx genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Susumu Seino (seino@med.kobe-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0475K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Biol Chem. 2008 283(8):4905-11.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data