RBRC03512

Information1

Image
BRC No.RBRC03512
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rspo2<tm1Hoka>/Rbrc
Former Common nameR-spondin2(lacZ) (Acc.No. CDB0467K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDev. Growth. Differ., 50, 85-95 (2008). 18067586

Health Report

Examination Date / Room / Rack2016/03/07Room:2-検Rack:E
2016/01/12Room:2-検Rack:E
2015/11/09Room:2-検Rack:E
2015/09/07Room:2-検Rack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rspo2R-spondin 2 homolog (Xenopus laevis)15Rspo2<tm1Hoka>targeted mutation 1, Hitoshi Okamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyA15HBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
LacZbeta-galactosidase (E. coli)15

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lox71mutant lox sequence15lox71

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 lox71, E. coli LacZ, poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase (Pgk) promoter, E. coli neo, phage P1 loxP, rabbit beta-globin polyA, mouse Rspo2 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Dr. Hitoshi Okamoto (hitoshi@brain.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0467K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Growth Differ. 2008 50(2):85-95.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data