Strain Data Sheet

RBRC03486

Strain Information

Image
BRC No.RBRC03486
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(NDE-hsp68-Nodal/MYC-tag,-lacZ)35Hmd
Former Common nameNDE-3 x Myc Nodal (35)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shinya Oki and Hiroshi Hamada, Osaka University in 2006.
Strain description
Colony maintenance
References
Sulfated glycosaminoglycans are necessary for Nodal signal transmission from the node to the left lateral plate in the mouse embryo.
Oki S, Hashimoto R, Okui Y, Shen M M, Mekada E, Otani H, Saijoh Y, Hamada H
Development., 134, 3893-3904 (2007). 17913787

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRESinternal ribosomal entry site (EMCV)UN
NodalnodalUNNodal
lacZbeta-galactosidase (E. coli)UN
myc-tagmyc-epitope tagUNmyc-tagmyelocytomatosis oncogene

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAマウス由来Nodalのノードエンハンサー, Nodal cDNA, hsp プロモーター, E.coli由来LacZ遺伝子, EMCV 由来由来タンパク合成開始シグナル(IRES), ヒトのc-Myc タンパク質の410〜419番目のアミノ酸部分 (合成ペプチド)
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development., 134, 3893-3904 (2007).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data