Strain Data Sheet

RBRC03482

Information1

Image
BRC No.RBRC03482
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Foxo3<tm1Nmt>
Former Common nameFoxo3a KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesCell Stem Cell, 1, 101-112 (2007). 18371339

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Foxo3forkhead box O310Foxo3<tm1Nmt>targeted mutation 1, Noboru Motoyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FlagFLAG tag (synthetic)10Flag

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit bata-globin polyA10HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP10loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNABacteriophage loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Phosphoglycerate kinase PolyA signal, FLAG-tag, mouse Foxo3a cDNA containing a triple Ala mutationat Thr32, Ser252 and Ser314 polyA, rabbit beta globin genomic DNA, mouse Foxo3 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Stem Cell, 1, 101-112 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Motoyama (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ni HM, Du K, You M, Ding WX.
Critical role of FoxO3a in alcohol-induced autophagy and hepatotoxicity.
Am J Pathol 183(6) 1815-1825(2013) 24095927