Strain Data Sheet

RBRC03482

Strain Information

Image
BRC No.RBRC03482
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Foxo3<tm1Nmt>
Former Common nameFoxo3a KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Foxo3a is essential for maintenance of the hematopoietic stem cell pool.
Miyamoto K, Araki K Y, Naka K, Arai F, Takubo K, Yamazaki S, Matsuoka S, Miyamoto T, Ito K, Ohmura M, Chen C, Hosokawa K, Nakauchi H, Nakayama K, Nakayama K I, Harada M, Motoyama N, Suda T, Hirao A
Cell Stem Cell, 1, 101-112 (2007). 18371339

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAGFLAG tag (synthetic)10FLAG
Foxo3forkhead box O310Foxo3targeted mutation 1, Noboru Motoyama
HBB2rabbit bata-globin polyA10HBB2
loxPphage P1 loxP10loxP
loxPphage P1 loxP10loxP
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal NK cell morphology(MP:0005068)

  • abnormal hematopoietic stem cell morphology(MP:0004808)

  • decreased apoptosis(MP:0006043)

  • decreased erythrocyte cell number(MP:0002875)

  • decreased hematopoietic stem cell number(MP:0004810)
    • more 8 phenotypes
  • decreased physiological sensitivity to xenobiotic(MP:0008874)

  • female infertility(MP:0001926)

  • hematopoietic system phenotype(MP:0005397)

  • increased NK cell number(MP:0008044)

  • increased mean corpuscular hemoglobin(MP:0005561)

  • increased mean corpuscular volume(MP:0002590)

  • increased susceptibility to xenobiotic induced morbidity/mortality(MP:0009766)

  • reticulocytosis(MP:0002640)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage loxP sites, mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Phosphoglycerate kinase PolyA signal, FLAG-tag, mouse Foxo3a cDNA containing a triple Ala mutationat Thr32, Ser252 and Ser314 polyA, rabbit beta globin genomic DNA, mouse Foxo3 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Stem Cell, 1, 101-112 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNoboru Motoyama (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Ni HM, Du K, You M, Ding WX.
    Critical role of FoxO3a in alcohol-induced autophagy and hepatotoxicity.
    Am J Pathol 183(6) 1815-1825(2013) 24095927