RBRC03460

Information1

Image
BRC No.RBRC03460
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Sgms1<tm1Kenw>
Former Common nameGOR2KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Biol. Chem., 286 (5) 3992-4002 (2011). 21115496

Health Report

Examination Date / Room / Rack2011/04/04Room:3-6Rack:B
2011/02/07Room:3-6Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sgms1sphingomyelin synthase 119Sgms1<tm1Kenw>targeted mutation 1, Ken Watanabe

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAbacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Sgms1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data