Strain Data Sheet

RBRC03459

Strain Information

Image
BRC No.RBRC03459
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Sgms1<tm1Kenw>
Former Common nameGOR2KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Ken Watanabe, Research Institute, National Center for Geriatrics and Gerontology.
Strain descriptionHomozygous mutant mice are embryonic or perinatal lethal.
Colony maintenance
References
Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice.
Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song W J, Node K, Taguchi R, Yamagata K, Oike Y
J. Biol. Chem., 286 (5) 3992-4002 (2011). 21115496

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sgms1sphingomyelin synthase 119Sgms1targeted mutation 1, Ken Watanabe
loxPphage P1 loxP19loxP
neoneomycin resistance gene (E. coli)19

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal pancreatic islet morphology(MP:0005215)

  • decreased body weight(MP:0001262)

  • decreased circulating insulin level(MP:0002727)

  • decreased insulin secretion(MP:0003059)

  • decreased white adipose tissue amount(MP:0001783)
    • more 5 phenotypes
  • hyperglycemia(MP:0001559)

  • impaired glucose tolerance(MP:0005293)

  • increased insulin sensitivity(MP:0002891)

  • oxidative stress(MP:0003674)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPGK プロモーター マウス, ネオマイシン耐性遺伝子 大腸菌, loxP エレメント bacteriophage
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data