Strain Data Sheet

RBRC03458

Strain Information

Image
BRC No.RBRC03458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Maged1<tm1Kenw>
Former Common nameDXN1KO, Maged1KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Dr. Ken Watanabe, Research Institute, National Center for Geriatrics and Gerontology. C57BL/6 background.
Strain descriptionMaged1 gene knockout mice. Exons 1 to 13 were replaced with a floxed neo cassette.
Colony maintenanceHeterozygote (female), Hemizygote (male) x Wild-type [C57BL/6JJcl]
References
MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation.
Mouri A, Sasaki A, Watanabe K, Sogawa C, Kitayama S, Mamiya T, Miyamoto Y, Yamada K, Noda Y, Nabeshima T
J. Neurosci., 28, 4562-4580 (2012). 22457503

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Maged1MAGE family member D1XMaged1targeted mutation 1, Ken Watanabe
lacZbeta-galactosidase (E. coli)X
loxPphage P1 loxPXloxP
neoneomycin resistance gene (E. coli)X

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal serotonin level(MP:0005322)

  • anhedonia(MP:0009710)

  • behavior/neurological phenotype(MP:0005386)

  • behavioral despair(MP:0002573)

  • decreased exploration in new environment(MP:0001417)
    • more 4 phenotypes
  • decreased locomotor activity(MP:0001402)

  • decreased serotonin level(MP:0010070)

  • increased serotonin level(MP:0010069)

  • social withdrawal(MP:0001361)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli Neomycin resistant gene, Mouse phosphoglycerate kinase promoter, Mouse MAGE-D1 gene, E. coli beta-galactosidase
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data