Strain Data Sheet

RBRC03456

Information1

Image
BRC No.RBRC03456
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6N.129S2-Lxn<tm1Yari>/YariRbrc
Former Common nameLxn KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Yasuyoshi Arimatsu, Mitsubishi Kagaku Institute of Life Sciences. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6N.
Strain descriptionLxn (latexin) gene knockout mice. Exon 1 of the Lxn gene was replaced with a neomycin resistant cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
ReferencesBrain Res., 1075(1):117-21 (2006). 16469302

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lxnlatexin3Lxn<tm1Yari>targeted mutation 1, Yasuyoshi Arimatsu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)3mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene,　mouse Lxn genomic DNA
Research application
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorYasuyoshi Arimatsu (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data