Strain Data Sheet

RBRC03451

Information1

Image
BRC No.RBRC03451
TypeTransgeneCartagena
SpeciesMus musculus
Strain namepOCT-GFP/BR Tg
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toshiaki Noce, Mitsubishi Kagaku Institute of Life Sciences.
Strain descriptionMvh (mouse Vasa homolog)-RFP and Oct4-GFP double transgenic mice.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Oct4 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RFPRed Fluorescent Protein (Discosoma sp.)UNRFPmouse vasa homolog promoter

Information2

Donor DNAMvh genomic DNA (mouse), RFP cDNA (Propionibacterium freudenreichi), Neomycin resistance gene (E. coli), Oct4 promoter (mouse), GFP cDNA (Jellyfish)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe BIOLOGICAL RESOURCE shall be used only for academic research purposes by a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical Corporation of RECIPIENT organization, RECIPIENT name, the specific research purpose and the date of distribution.
DepositorTosiaki Nose (Mitsubishi Kasei Institute of Life Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data